3/2/2023 0 Comments You to the tube![]() ![]() The specified anticoagulant or preservative must be used for the test ordered. Both anticoagulants and preservatives may be required for plasma specimens. Several different types of anticoagulants interfere with the activity of these factors to prevent clotting. There are numerous coagulation factors (factor VIII, factor IX, etc) involved in the clotting of blood. This mixed blood may then be centrifuged, yielding plasma, which contains albumin, globulin, and fibrinogen. Plasma is obtained from blood that has been mixed with an anticoagulant in the collection tube and has, therefore, not clotted. Serum is usually collected in mottled red/gray, gold, or cherry red-top tubes, and red-top tubes are occasionally used. This clotted blood is then centrifuged, yielding serum, which contains two types of protein: albumin and globulin. Serum is obtained from clotted blood that has not been mixed with an anticoagulant (a chemical that prevents the clotting of blood). The essential difference between plasma and serum is that plasma retains fibrinogen (the clotting component), which is removed from serum. Platelets are small fragments of special cells that aid in blood clotting.Įither plasma or serum may be separated from the blood cells by centrifugation. In a healthy person, the white cells respond to minor infections by increasing in number and eliminating pathogens. ![]() The primary purpose of the white cells is to fight infection. Hemolysis can be caused by rough handling of a blood specimen, leaving the tourniquet on too long (causing blood stasis) or squeezing the tip of the finger too hard during capillary collection, dilution, exposure to contaminants, extremes in temperature, or pathologic conditions. Hemolysis occurs when the thin protective membrane that encases the fragile red cells is ruptured, allowing hemoglobin to escape into the plasma. The red cells are delicate, round, concave bodies that contain hemoglobin, the complex chemical that transports oxygen and carbon dioxide. The major blood cells are classified as red cells (erythrocytes), white cells (leukocytes), and platelets (thrombocytes). ![]() In the average adult male there are approximately 5 quarts (4.75 liters) of blood, composed of about 3 quarts (2.85 liters) of plasma and 2 quarts (1.9 liters) of cells.īlood cells are suspended in the plasma, which is made up of water and dissolved materials, including hormones, antibodies, and enzymes that are being carried to the tissues, and cellular waste products that are being carried to the lungs and kidneys. (See specific Microbiology Specimen sections for additional instructions.) Blood Components ![]()
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